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Evonik
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Funakoshi ltd
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Evonik
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Image Search Results
Journal: Oncology Letters
Article Title: Enhanced expression of hydroxylated ceramide in well-differentiated endometrial adenocarcinoma
doi: 10.3892/ol.2016.5410
Figure Lengend Snippet: Thin-layer chromatography of neutral glycolipids from human endometrial adenocarcinoma of various degrees of differentiation. The neutral glycolipids obtained from cancerous tissues (lanes 1–18) were chromatographed with chloroform/methanol/water (65:35:8, v/v) and located by spraying orcinol-H 2 S0 4 reagent and heating at 110°C for 10 min. Lanes 1–9 are well-differentiated adenocarcinoma tissues, while lanes 10–14 and 15–18 correspond to moderately and poorly differentiated adenocarcinoma tissues, respectively. GlcCer, glucosylceramide; LacCer, lactosylceramide; Gb 3 Cer, globotriaosylceramide; Gb 4 Cer, globotetraosylceramide.
Article Snippet: Standard glycolipids (
Techniques: Thin Layer Chromatography
Journal: Oncology Letters
Article Title: Enhanced expression of hydroxylated ceramide in well-differentiated endometrial adenocarcinoma
doi: 10.3892/ol.2016.5410
Figure Lengend Snippet: (A) TLC of the constituents of the Gb 3 Cer and Gb 4 Cer bands isolated from well- (bands T1 and G1) and poorly (bands T2 and G2) differentiated endometrial adenocarcinoma. Neutral glycolipids were isolated from the total neutral lipid fraction as described in Materials and methods. The total neutral glycolipids and the isolated glycolipids were developed with chloroform/methanol/water (65:35:8, v/v) and visualized with orcinol-H 2 S0 4 reagent. Lanes Well and Poorly correspond to the total neutral glycolipids in well- and poorly differentiated adenocarcinoma, respectively. Lanes T1, T2, G1 and G2 are the isolated neutral glycolipids from the three bands of Gb 3 Cer and the band of Gb 4 Cer, respectively. (B) TLC schema of T1, T2, G1 and G2. TLC, thin-layer chromatography; GlcCer, glucosylceramide; LacCer, lactosylceramide; Gb 3 Cer, globotriaosylceramide; Gb 4 Cer, globotetraosylceramide; Lc 4 Cer, lactotetraosyl ceramide.
Article Snippet: Standard glycolipids (
Techniques: Isolation, Thin Layer Chromatography
Journal: Oncology Letters
Article Title: Enhanced expression of hydroxylated ceramide in well-differentiated endometrial adenocarcinoma
doi: 10.3892/ol.2016.5410
Figure Lengend Snippet: Relative expression of each band of glucosylceramide, lactosylceramide, globotriaosylceramide and globotetraosylceramide in well-, moderately and poorly differentiated adenocarcinoma of the endometrium. Bands containing phytosphingosine in Gb 3 Cer and Gb 4 Cer of well differentiated adenocarcinoma are designated as black closed column. The relative amount of this characteristic band was shown. GlcCer, glucosylceramide; LacCer, lactosylceramide; Gb 3 Cer, globotriaosylceramide; Gb 4 Cer, globotetraosylceramide.
Article Snippet: Standard glycolipids (
Techniques: Expressing
Journal: Oncology Letters
Article Title: Enhanced expression of hydroxylated ceramide in well-differentiated endometrial adenocarcinoma
doi: 10.3892/ol.2016.5410
Figure Lengend Snippet: (A) Structural analysis of the sugar chains of LacCer, as indicated by arrows, by TLC following enzyme treatment with α-galactosidase. Upon treatment with the enzyme, both T1 and T2 changed to LacCer, and both were observed to be Gb 3 Cer with a terminal α-galactose. (B) Analysis of the composition of the long-chain base by TLC. Dihydrosphingosine was noticed to be the principal component in both T1 and T2, while sphingosine or phytosphingosine were not detected in either of them. However, TLC analysis revealed that the ceramide of G1 was composed of sphingosine, dihydrosphingosine and phytosphingosine, while G2 contained just sphingosine and dihydrosphingosine. TLC, thin-layer chromatography; LacCer, lactosylceramide; Gb 3 Cer, globotriaosylceramide.
Article Snippet: Standard glycolipids (
Techniques: Thin Layer Chromatography